What does TNI stand for?

TNI stands for the “manipulation of gene expression by Targeted Nutritional Intervention,” a description coined by W. James Croom, Phd. NCSU, research scientist and team member.

Is it possible to regulate proteins and enzymes through targeted supplements?

Yes. Peer Reviewed, published trials prove that gene expression can be manipulated with nutrients and natural substances.

Who developed Targeted Nutritional Intervention?

A team of this country’s top research scientists put the theory of TNI to test and formulated the original TNI in 1993. It is still monitored today and modified by scientists as continued research dictates.

Is this a cure for DS?

No, it is a way to safely manage our children’s unique biochemical needs to enable them to reach their full potential as healthy, active, able individuals.

Do all people with DS need this?

Yes because all people with DS have altered biochemistry due to gene over expression.

Have any studies been completed?

Over the years each major component has been studied numerous times and published in peer reviewed journals. The entire protocol has been through two third-party trials. One was completed in 2001 by Mathias Gelb and published in the German Journal of Pediatrics. A second trial followed shortly after authored by N. Miguid at the University of Cairo, Egypt. See research section For copies.

What were the results?

Blood tests, growth parameters, immune response and cognitive function tests demonstrated that TNI is effective.

Did the researchers share data during the trial periods?

No. They were complete strangers unaware that another trial was being conducted.

Were the researchers associated with the TNI team or International Nutrition?

No, these were independent studies by unbiased reporters.

How does TNI work?

The human genome relies on foods to function, not drugs. Nature has thankfully provided numerous substances that, when provided in the correct doses, from appropriate sources, can inhibit or down regulate over expressed genes, These have been studied both in vitro and in vivo. The genes mapped to the DS critical region of the additional 21st chromosome are actively adding their product (proteins, enzymes, etc.) to the patients cells causing a disruption of a large portion of the genome. These genes result in the phenotypic features (physical and neurological) of Down syndrome. If these genes were inactive, as are those on the rest of the additional chromosome, your child would not have Down syndrome. It is the active genes that produce the anomalies associated with DS.

TNI uses substances studied for their ability to down regulate or inhibit these genes. Because the presence of so many additional proteins disrupts numerous pathways and other proteins, TNI also regulates these pathways. Let’s look at just one example.

DYRK1a – this gene is a dual kinase. It is active in DS and therefore hyperphosforlates proteins that are not even located on C21. It is the job of DYRK1a to phosphorlate proteins, but when too much is present, the proteins are damaged. This causes numerous proteins to malfunction and in turn, everything that utilizes these hyperphosforlated proteins is disrupted. This greatly contributes to cognitive delay and the development of Alzheimer’s pathology. How do we address this gene? EGCG, a catichin found in green tea and some fruits and vegetables, is a known DYRK1a inhibitor. This inhibitor has been studied in the DS mouse model and in human beings with Down syndrome. All studies were very successful.

EGCG also inhibits several other genes that are over expressed in Down syndrome. This makes its use very important. However, in using EGCG caution must be taken to dose it only as high as necessary to inhibit these genes. After careful review of Blood profiles, including liver enzymes, folate, T Cells and iron in DS, the dosage is fixed at 5 mg per kg. At this dose genes are inhibited with no potential for side effects,

The entire protocol is designed to down regulate the most dangerous active genes and to normalize DS biochemistry to the extent presently possible. The results are excellent.

It is important to know that EGCG alone will not down regulate enough genes to significantly normalize your child’s biochemistry. Far too many other genes and proteins over expressed in DS are not effected by EGCG. Every ingredient in the Nutrivene protocol is designed to regulate genes and proteins, support  other ingredients and to work in synergy for optimal results.

Is TNI harmful?

Targeted Nutritional Intervention has been in use worldwide for 24 years. No child has ever suffered any uncomfortable or serious side effects. This is not a drug regimine. It only contains natural substances in amounts necessary to produce the desired effect without causing harm. There are thousands of patients worldwide who have been on TNI for the entire 24 years, long term use is not harmful.

 Because it does alter your child biochemistry, it is important to slowly introduce it. Your child will need time to adjust.

What goes into the protocol.

Pharmaceutical grade ingredients are utilized in compounding Nutrivene. This insures that they are safe and effective.

What safety measures are taken during development?

Nutrivene is produced in an FDA Certified laboratory and is repeatedly tested under those standards for all potential contaminants including heavy metals. It is non GMO and manufactured under GMP.

Epigallocatechin-3-gallate, a DYRK1A inhibitor, rescues cognitive deficits in Down syndrome mouse models and in humans.